PrimePCR™ SYBR® Green Assay: CABYR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031961

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CABYR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CABYR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018616
Assay Design:   Intron-spanning
Chromosome Location:   18:21740003-21741546question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000399496 ENST00000327201 ENST00000415309 ENST00000399499

Gene Information

To reach fertilization competence spermatozoa undergo a series of morphological and molecular maturational processes termed capacitation involving protein tyrosine phosphorylation and increased intracellular calcium. The protein encoded by this gene localizes to the principal piece of the sperm flagellum in association with the fibrous sheath and exhibits calcium-binding when phosphorylated during capacitation. A pseudogene on chromosome 3 has been identified for this gene. Transcript variants of this gene encode multiple protein isoforms. An additional transcript and isoform has not been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   CABYR
Gene Name:   calcium binding tyrosine-(Y)-phosphorylation regulated
Aliases:   CABYRa, CABYRc, CABYRc/d, CBP86, CT88, FSP-2, FSP2, MGC9117
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000154040
Entrez:   26256
Chromosome Mapping:   18q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.560000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download