PrimePCR™ SYBR® Green Assay: ADAM9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018553
Assay Design:   Intron-spanning
Chromosome Location:   8:38865405-38871501question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000466936 ENST00000481513 ENST00000487273

Gene Information

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions including fertilization muscle development and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins binds mitotic arrest deficient 2 beta protein and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   ADAM9
Gene Name:   ADAM metallopeptidase domain 9
Aliases:   CORD9, KIAA0021, MCMP, MDC9, Mltng
RefSeq:   NC_000008.10 NG_016335.1 NT_167187.1
Ensembl:   ENSG00000168615
Entrez:   8754
UniGene:   Hs.591852
Chromosome Mapping:   8p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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