PrimePCR™ SYBR® Green Assay: ABCB5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031906

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ABCB5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ABCB5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018521
Assay Design:   Intron-spanning
Chromosome Location:   7:20689700-20691137question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000404938 ENST00000443026 ENST00000406935 ENST00000258738

Gene Information

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions sugars and peptides to more complex organic molecules (Chen et al. 2005 [PubMed 15760339]).[supplied by OMIM Mar 2008]

Gene Symbol:   ABCB5
Gene Name:   ATP-binding cassette, sub-family B (MDR/TAP), member 5
Aliases:   ABCB5alpha, ABCB5beta, EST422562
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000004846
Entrez:   340273
Chromosome Mapping:   7p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download