PrimePCR™ SYBR® Green Assay: DTL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031786

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018324
Assay Design:   Intron-spanning
Chromosome Location:   1:212254013-212273681question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000366991 ENST00000542077 ENST00000420235

Gene Information

Description Not Available

Gene Symbol:   DTL
Gene Name:   denticleless homolog (Drosophila)
Aliases:   CDT2, DCAF2, L2DTL, RAMP
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143476
Entrez:   51514
UniGene:   Hs.656473
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.640000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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