PrimePCR™ SYBR® Green Assay: TTF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031782

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018316
Assay Design:   Intron-spanning
Chromosome Location:   1:117641455-117644031question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000369466 ENST00000427271

Gene Information

This gene encodes a member of the SWI2/SNF2 family of proteins which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like associates with human splicing complexes and plays a role in pre-mRNA splicing. [provided by RefSeq Jul 2008]

Gene Symbol:   TTF2
Gene Name:   transcription termination factor, RNA polymerase II
Aliases:   HuF2
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000116830
Entrez:   8458
UniGene:   Hs.486818
Chromosome Mapping:   1p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.420000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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