PrimePCR™ SYBR® Green Assay: NMD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031779

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NMD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NMD3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018308
Assay Design:   Intron-spanning
Chromosome Location:   3:160951218-160952586question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000460503 ENST00000493066 ENST00000351193 ENST00000472947 ENST00000463518 ENST00000476237 ENST00000460469 ENST00000540137

Gene Information

Ribosomes are composed of 60S and 40S subunits that are assembled in the nucleolus and exported to the cytoplasm through nuclear pore complexes in the nuclear envelope. NMD3 is an adaptor for 60S subunit export via the CRM1 (XPO1; MIM 602559) pathway (Trotta et al. 2003 [PubMed 12773398]).[supplied by OMIM Mar 2008]

Gene Symbol:   NMD3
Gene Name:   NMD3 homolog (S. cerevisiae)
Aliases:   FLJ21053
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000169251
Entrez:   51068
Chromosome Mapping:   3q26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 35.500000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download