PrimePCR™ SYBR® Green Assay: DCP1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031687

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018155
Assay Design:   Intron-spanning
Chromosome Location:   12:2102481-2107133question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000280665 ENST00000541700 ENST00000535873 ENST00000397173

Gene Information

DCP1B is a core component of the mRNA decapping complex a key factor in the regulation of mRNA decay (Lykke-Andersen 2002 [PubMed 12417715]).[supplied by OMIM Mar 2008]

Gene Symbol:   DCP1B
Gene Name:   DCP1 decapping enzyme homolog B (S. cerevisiae)
Aliases:   DCP1, hDcp1b
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000151065
Entrez:   196513
UniGene:   Hs.130934
Chromosome Mapping:   12p13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.000000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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