PrimePCR™ SYBR® Green Assay: BROX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018154
Assay Design:   Intron-spanning
Chromosome Location:   1:222897444-222898874question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000340934 ENST00000426638 ENST00000539697 ENST00000537020

Gene Information

Description Not Available

Gene Symbol:   BROX
Gene Name:   BRO1 domain and CAAX motif containing
Aliases:   C1orf58, FLJ32421, MGC142195, MGC142197, RP11-452F19.1
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000162819
Entrez:   148362
UniGene:   Hs.552608
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.820000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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