PrimePCR™ SYBR® Green Assay: FIG4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: FIG4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FIG4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018137
Assay Design:   Intron-spanning
Chromosome Location:   6:110053841-110056397question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000230124 ENST00000454215 ENST00000368941 ENST00000441478

Gene Information

The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain approximately 400 amino acids in length and consisting of seven conserved motifs has been shown to possess phosphoinositide phosphatase activity. The yeast homolog Sac1p is involved in the regulation of various phosphoinositides and affects diverse cellular functions such as actin cytoskeleton organization Golgi function and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease type 4J. [provided by RefSeq Jul 2008]

Gene Symbol:   FIG4
Gene Name:   FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae)
Aliases:   ALS11, CMT4J, KIAA0274, SAC3, dJ249I4.1
RefSeq:   NC_000006.11 NG_007977.1 NT_025741.15
Ensembl:   ENSG00000112367
Entrez:   9896
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.270000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download