PrimePCR™ SYBR® Green Assay: DOCK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031654

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DOCK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DOCK2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018110
Assay Design:   Intron-spanning
Chromosome Location:   5:169230124-169267794question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000256935 ENST00000520908 ENST00000519628 ENST00000343291 ENST00000540750

Gene Information

The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells predominantly in the peripheral blood leukocytes and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq Oct 2011]

Gene Symbol:   DOCK2
Gene Name:   dedicator of cytokinesis 2
Aliases:   FLJ46592, KIAA0209
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000134516
Entrez:   1794
Chromosome Mapping:   5q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 33.870000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download