PrimePCR™ SYBR® Green Assay: MYO5A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031644

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MYO5A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MYO5A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018091
Assay Design:   Intron-spanning
Chromosome Location:   15:52681459-52684175question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000399231 ENST00000399229 ENST00000553916 ENST00000399233 ENST00000356338 ENST00000358212 ENST00000546028

Gene Information

This gene is one of three myosin V heavy-chain genes belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1) Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported but the full-length nature of some variants has not been determined. [provided by RefSeq Dec 2008]

Gene Symbol:   MYO5A
Gene Name:   myosin VA (heavy chain 12, myoxin)
Aliases:   GS1, MYH12, MYO5, MYR12
RefSeq:   NC_000015.9 NG_009887.1 NT_010194.17
Ensembl:   ENSG00000197535
Entrez:   4644
Chromosome Mapping:   15q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download