PrimePCR™ SYBR® Green Assay: NPHP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NPHP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NPHP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018072
Assay Design:   Intron-spanning
Chromosome Location:   3:132402341-132403466question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000512094 ENST00000337331 ENST00000393144 ENST00000393156 ENST00000326682

Gene Information

This gene encodes a protein containing a coiled-coil (CC) domain a tubulin-tyrosine ligase (TTL) domain and a tetratrico peptide repeat (TPR) domain. The encoded protein interacts with nephrocystin it is required for normal ciliary development and it functions in renal tubular development. Mutations in this gene are associated with nephronophthisis type 3 and also with renal-hepatic-pancreatic dysplasia and Meckel syndrome type 7. Naturally occurring read-through transcripts exist between this gene and the downstream ACAD11 (acyl-CoA dehydrogenase family member 11) gene. [provided by RefSeq Feb 2011]

Gene Symbol:   NPHP3
Gene Name:   nephronophthisis 3 (adolescent)
Aliases:   DKFZp667K242, DKFZp781K1312, FLJ30691, FLJ36696, KIAA2000, MGC78666, MKS7, NPH3, RHPD
RefSeq:   NC_000003.11 NG_008130.1 NT_005612.16
Ensembl:   ENSG00000113971
Entrez:   27031
Chromosome Mapping:   3q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.410000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download