PrimePCR™ SYBR® Green Assay: ATP11B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031630

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP11B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP11B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018071
Assay Design:   Intron-spanning
Chromosome Location:   3:182605455-182607280question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000323116 ENST00000498086 ENST00000482070 ENST00000442545

Gene Information

P-type ATPases such as ATP11B are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions such as H(+) Na(+) K(+) or Ca(+). A third subfamily transports amphipaths such as phosphatidylserine.[supplied by OMIM Feb 2005]

Gene Symbol:   ATP11B
Gene Name:   ATPase, class VI, type 11B
Aliases:   ATPIF, ATPIR, DKFZp434J238, DKFZp434N1615, KIAA0956, MGC46576
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000058063
Entrez:   23200
Chromosome Mapping:   3q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.020000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download