PrimePCR™ SYBR® Green Assay: FMNL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031583

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017981
Assay Design:   Intron-spanning
Chromosome Location:   2:153483191-153484897question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000288670 ENST00000475377 ENST00000421344

Gene Information

This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis cytokinesis and cell polarity. Alternatively spliced transcript variants encoding different isoforms have been described but their full-length nature has yet to be determined. [provided by RefSeq Jul 2008]

Gene Symbol:   FMNL2
Gene Name:   formin-like 2
Aliases:   FHOD2, FLJ37546
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000157827
Entrez:   114793
UniGene:   Hs.654630
Chromosome Mapping:   2q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.590000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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