PrimePCR™ SYBR® Green Assay: WDR48, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031556

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017943
Assay Design:   Intron-spanning
Chromosome Location:   3:39108320-39110322question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000302313 ENST00000441361 ENST00000396258 ENST00000418020

Gene Information

Description Not Available

Gene Symbol:   WDR48
Gene Name:   WD repeat domain 48
Aliases:   DKFZp686G1794, KIAA1449, P80, UAF1
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000114742
Entrez:   57599
UniGene:   Hs.109778
Chromosome Mapping:   3p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.800000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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