PrimePCR™ SYBR® Green Assay: TAX1BP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031542

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TAX1BP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TAX1BP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017922
Assay Design:   Intron-spanning
Chromosome Location:   7:27788214-27797733question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000418691 ENST00000422800 ENST00000265393 ENST00000409980 ENST00000396319 ENST00000543117 ENST00000433216

Gene Information

This gene encodes a HTLV-1 tax1 binding protein. The encoded protein interacts with TNFAIP3 and inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. Degradation of this protein by caspase-3-like family proteins is associated with apoptosis induced by TNF. This protein may also have a role in the inhibition of inflammatory signaling pathways. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq May 2011]

Gene Symbol:   TAX1BP1
Gene Name:   Tax1 (human T-cell leukemia virus type I) binding protein 1
Aliases:   CALCOCO3, T6BP, TXBP151
RefSeq:   NC_000007.13 NG_011499.1 NT_007819.17
Ensembl:   ENSG00000106052
Entrez:   8887
Chromosome Mapping:   7p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download