This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031524
PrimePCR™ PreAmp for SYBR® Green Assay: DNAH5, Human
PrimePCR™ Template for SYBR® Green Assay: DNAH5, Human
This gene encodes a dynein protein which is part of a microtubule-associated motor protein complex consisting of heavy light and intermediate chains. This protein is an axonemal heavy chain dynein. It functions as a force-generating protein with ATPase activity whereby the release of ADP is thought to produce the force-producing power stroke. Mutations in this gene cause primary ciliary dyskinesia type 3 as well as Kartagener syndrome which are both diseases due to ciliary defects. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.