PrimePCR™ SYBR® Green Assay: IPP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031520

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017881
Assay Design:   Intron-spanning
Chromosome Location:   1:46206907-46211852question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000359942 ENST00000396478

Gene Information

The protein encoded by this gene is a member of the kelch family of proteins which is characterized by a 50 amino acid repeat which interacts with actin. Transcript variants have been described but their full-length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   IPP
Gene Name:   intracisternal A particle-promoted polypeptide
Aliases:   KLHL27
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000197429
Entrez:   3652
UniGene:   Hs.699548
Chromosome Mapping:   1p34-p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 34.500000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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