PrimePCR™ SYBR® Green Assay: ACADL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017845
Assay Design:   Intron-spanning
Chromosome Location:   2:211082761-211085446question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000233710

Gene Information

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency leading to nonketotic hypoglycemia. [provided by RefSeq Jul 2008]

Gene Symbol:   ACADL
Gene Name:   acyl-CoA dehydrogenase, long chain
Aliases:   ACAD4, FLJ94052, LCAD
RefSeq:   NC_000002.11 NG_008002.1 NT_005403.17
Ensembl:   ENSG00000115361
Entrez:   33
UniGene:   Hs.471277
Chromosome Mapping:   2q34-q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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