PrimePCR™ SYBR® Green Assay: BCAS3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031491

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BCAS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BCAS3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017840
Assay Design:   Intron-spanning
Chromosome Location:   17:58987952-59001826question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000390652 ENST00000407086 ENST00000408905 ENST00000360207 ENST00000405070 ENST00000405217

Gene Information

Description Not Available

Gene Symbol:   BCAS3
Gene Name:   breast carcinoma amplified sequence 3
Aliases:   DKFZp686O1527, FLJ20128, GAOB1, MAAB, MGC4973
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000141376
Entrez:   54828
Chromosome Mapping:   17q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download