PrimePCR™ SYBR® Green Assay: NUDT12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031455

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NUDT12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NUDT12, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017781
Assay Design:   Intron-spanning
Chromosome Location:   5:102890493-102891676question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000230792 ENST00000507423

Gene Information

Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates cofactors and effectors. Nudix hydrolases such as NUDT12 regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al. 2003 [PubMed 12790796]).[supplied by OMIM Mar 2008]

Gene Symbol:   NUDT12
Gene Name:   nudix (nucleoside diphosphate linked moiety X)-type motif 12
Aliases:   DKFZp761I172
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000112874
Entrez:   83594
Chromosome Mapping:   5q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.030000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download