PrimePCR™ SYBR® Green Assay: SLC25A21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017655
Assay Design:   Intron-spanning
Chromosome Location:   14:37198745-37203776question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000555449 ENST00000331299

Gene Information

SLC25A21 is a homolog of the S. cerevisiae ODC proteins mitochondrial carriers that transport C5-C7 oxodicarboxylates across inner mitochondrial membranes. One of the species transported by ODC is 2-oxoadipate a common intermediate in the catabolism of lysine tryptophan and hydroxylysine in mammals. Within mitochondria 2-oxoadipate is converted into acetyl-CoA.[supplied by OMIM Apr 2004]

Gene Symbol:   SLC25A21
Gene Name:   solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21
Aliases:   MGC126570, ODC, ODC1
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000183032
Entrez:   89874
UniGene:   Hs.730857
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.630000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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