PrimePCR™ SYBR® Green Assay: SLC47A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031374

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017630
Assay Design:   Intron-spanning
Chromosome Location:   17:19459340-19463577question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000270570 ENST00000395585 ENST00000457293 ENST00000436810 ENST00000542886 ENST00000455670 ENST00000424755

Gene Information

This gene is located within the Smith-Magenis syndrome region on chromosome 17. It encodes a protein of unknown function. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC47A1
Gene Name:   solute carrier family 47, member 1
Aliases:   FLJ10847, MATE1, MGC64822
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000142494
Entrez:   55244
UniGene:   Hs.232054
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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