This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031308
PrimePCR™ PreAmp for SYBR® Green Assay: TDRD7, Human
PrimePCR™ Template for SYBR® Green Assay: TDRD7, Human
This gene encodes a component of cytoplasmic RNA granules which are involved in determining the fate of mRNAs. Mutation in this gene has been associated with pediatric cataracts. Mutation of the similar gene in mice has been associated with cataracts glaucoma and a block in spermatogenesis. [provided by RefSeq May 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.