PrimePCR™ SYBR® Green Assay: ABHD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031286

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017505
Assay Design:   Intron-spanning
Chromosome Location:   18:19237065-19239247question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000289119

Gene Information

This gene encodes a protein containing an alpha/beta hydrolase fold which is a catalytic domain found in a very wide range of enzymes. The function of this protein has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   ABHD3
Gene Name:   abhydrolase domain containing 3
Aliases:   LABH3, MGC11259
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000158201
Entrez:   171586
Chromosome Mapping:   18q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.440000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download