PrimePCR™ SYBR® Green Assay: HTRA4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031276

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HTRA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HTRA4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017484
Assay Design:   Intron-spanning
Chromosome Location:   8:38840496-38845501question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000302495

Gene Information

This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide an insulin growth factor binding domain a Kazal protease inhibitor domain a conserved trypsin domain and a PDZ domain. Based on studies on other related family members this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis tumor suppression unfolded stress response apoptosis and aging. [provided by RefSeq Oct 2008]

Gene Symbol:   HTRA4
Gene Name:   HtrA serine peptidase 4
Aliases:   FLJ90724
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000169495
Entrez:   203100
Chromosome Mapping:   8p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.810000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download