This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031112
PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP17, Human
PrimePCR™ Template for SYBR® Green Assay: ARHGAP17, Human
RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins such as RHOA (MIM 165390) RAC1 (MIM 602048) and CDC42 (MIM 116952).[supplied by OMIM Apr 2004]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.