PrimePCR™ SYBR® Green Assay: EDEM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031067

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017177
Assay Design:   Intron-spanning
Chromosome Location:   20:33714112-33719525question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000374492 ENST00000374491 ENST00000541621 ENST00000542871 ENST00000540582

Gene Information

In the endoplasmic reticulum (ER) misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al. 2005 [PubMed 15537790]).[supplied by OMIM Mar 2008]

Gene Symbol:   EDEM2
Gene Name:   ER degradation enhancer, mannosidase alpha-like 2
Aliases:   C20orf31, C20orf49, FLJ10783, bA4204.1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000088298
Entrez:   55741
UniGene:   Hs.720177
Chromosome Mapping:   20q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.280000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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