PrimePCR™ SYBR® Green Assay: RFX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017048
Assay Design:   Intron-spanning
Chromosome Location:   19:6002753-6004260question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000303657 ENST00000359161 ENST00000537791

Gene Information

This gene is a member of the regulatory factor X gene family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1 X3 X4 and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene and both variants utilize alternative polyadenylation sites. [provided by RefSeq Jul 2008]

Gene Symbol:   RFX2
Gene Name:   regulatory factor X, 2 (influences HLA class II expression)
Aliases:   FLJ14226
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000087903
Entrez:   5990
UniGene:   Hs.465709
Chromosome Mapping:   19p13.3-p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.160000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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