PrimePCR™ SYBR® Green Assay: BRD4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030984

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BRD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BRD4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017046
Assay Design:   Intron-spanning
Chromosome Location:   19:15379778-15383708question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000263377 ENST00000371835 ENST00000360016

Gene Information

The protein encoded by this gene is homologous to the murine protein MCAP which associates with chromosomes during mitosis and to the human RING3 protein a serine/threonine kinase. Each of these proteins contains two bromodomains a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1) which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   BRD4
Gene Name:   bromodomain containing 4
Aliases:   CAP, HUNK1, HUNKI, MCAP
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000141867
Entrez:   23476
Chromosome Mapping:   19p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.320000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download