PrimePCR™ SYBR® Green Assay: APH1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030878

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016895
Assay Design:   Intron-spanning
Chromosome Location:   15:63571398-63578797question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000261879 ENST00000380343 ENST00000380340

Gene Information

This gene encodes a multi-pass transmembrane protein that is a functional component of the gamma-secretase complex which also contains presenilin and nicastrin. This protein represents a stabilizing cofactor for the presenilin holoprotein in the complex. The gamma-secretase complex catalyzes the cleavage of integral proteins such as notch receptors and beta-amyloid precursor protein. [provided by RefSeq Sep 2011]

Gene Symbol:   APH1B
Gene Name:   anterior pharynx defective 1 homolog B (C. elegans)
Aliases:   APH-1B, DKFZp564D0372, FLJ33115, PRO1328, PSFL, TAAV688
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000138613
Entrez:   83464
UniGene:   Hs.511703
Chromosome Mapping:   15q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.260000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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