PrimePCR™ SYBR® Green Assay: RNASEH2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030875

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RNASEH2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RNASEH2A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016890
Assay Design:   Intron-spanning
Chromosome Location:   19:12918235-12920921question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000221486

Gene Information

The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS) a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation intracranial calcifications elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq Aug 2009]

Gene Symbol:   RNASEH2A
Gene Name:   ribonuclease H2, subunit A
Aliases:   AGS4, JUNB, RNASEHI, RNHIA, RNHL
RefSeq:   NC_000019.9 NG_012662.1 NT_011295.11
Ensembl:   ENSG00000104889
Entrez:   10535
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.410000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download