PrimePCR™ SYBR® Green Assay: COBRA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030813

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: COBRA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COBRA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016797
Assay Design:   Intron-spanning
Chromosome Location:   9:140157616-140158797question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000343053

Gene Information

NELFB is a subunit of negative elongation factor (NELF) which also includes NELFA (WHSC2; MIM 606026) either NELFC or NELFD (TH1L; MIM 605297) and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF) a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102) to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al. 2003 [PubMed 12612062]).[supplied by OMIM Mar 2008]

Gene Symbol:   COBRA1
Gene Name:   cofactor of BRCA1
Aliases:   DKFZp586B0519, KIAA1182, NELF-B, NELFB
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000188986
Entrez:   25920
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.270000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download