PrimePCR™ SYBR® Green Assay: ST6GALNAC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016785
Assay Design:   Intron-spanning
Chromosome Location:   17:74562288-74563626question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000225276

Gene Information

ST6GALNAC2 belongs to a family of sialyltransferases that add sialic acids to the nonreducing ends of glycoconjugates. At the cell surface these modifications have roles in cell-cell and cell-substrate interactions bacterial adhesion and protein targeting (Samyn-Petit et al. 2000 [PubMed 10742600]).[supplied by OMIM Mar 2008]

Gene Symbol:   ST6GALNAC2
Gene Name:   ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
Aliases:   FLJ45660, SAITL1, SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000070731
Entrez:   10610
UniGene:   Hs.592105
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.410000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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