PrimePCR™ SYBR® Green Assay: SASS6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030796

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016775
Assay Design:   Intron-spanning
Chromosome Location:   1:100573487-100575927question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000287482 ENST00000539329 ENST00000535161

Gene Information

SAS6 is necessary for centrosome duplication and functions during procentriole formation; SAS6 functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle Strnad et al. (2007) [PubMed 17681132].[supplied by OMIM Mar 2008]

Gene Symbol:   SASS6
Gene Name:   spindle assembly 6 homolog (C. elegans)
Aliases:   FLJ22097, MGC119440, SAS-6, SAS6
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000156876
Entrez:   163786
UniGene:   Hs.591447
Chromosome Mapping:   1p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995200
y-intercept 35.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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