This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SCGB1D1, Human
PrimePCR™ Template for SYBR® Green Assay: SCGB1D1, Human
The protein encoded by this gene is a member of the lipophilin subfamily part of the uteroglobin superfamily and is an ortholog of prostatein the major secretory glycoprotein of the rat ventral prostate gland. This gene product represents one component of a heterodimeric molecule present in human tears whose elution profile is consistent with prostatein a tetrameric molecule composed of three peptide components in heterodimers. Assuming that human lipophilins are the functional counterparts of prostatein they may be transcriptionally regulated by steroid hormones with the ability to bind androgens other steroids and possibly bind and concentrate estramustine a chemotherapeutic agent widely used for prostate cancer. Although the gene has been reported to be on chromosome 15 this sequence appears to be from a cluster of genes on chromosome 11 that includes mammaglobin 2. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.