This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030748
PrimePCR™ PreAmp for SYBR® Green Assay: SLC18A2, Human
PrimePCR™ Template for SYBR® Green Assay: SLC18A2, Human
The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs including reserpine and tetrabenazine (summary by Peter et al. 1993 [PubMed 7905859]). See also SLC18A1 (MIM 193002).[supplied by OMIM Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.