PrimePCR™ SYBR® Green Assay: PARN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030692

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016600
Assay Design:   Intron-spanning
Chromosome Location:   16:14722060-14723511question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000437198 ENST00000420015 ENST00000539279 ENST00000341484 ENST00000538472

Gene Information

The protein encoded by this gene is a 3'-exoribonuclease with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate hence efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:   PARN
Gene Name:   poly(A)-specific ribonuclease (deadenylation nuclease)
Aliases:   DAN
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000140694
Entrez:   5073
UniGene:   Hs.253197
Chromosome Mapping:   16p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 34.790000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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