PrimePCR™ SYBR® Green Assay: AQR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030653

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016538
Assay Design:   Intron-spanning
Chromosome Location:   15:35149195-35152304question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000543879 ENST00000156471

Gene Information

Description Not Available

Gene Symbol:   AQR
Gene Name:   aquarius homolog (mouse)
Aliases:   DKFZp686B23123, IBP160, KIAA0560, fSAP164
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000021776
Entrez:   9716
UniGene:   Hs.510958
Chromosome Mapping:   15q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.440000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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