PrimePCR™ SYBR® Green Assay: SEPT12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030649

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016533
Assay Design:   Intron-spanning
Chromosome Location:   16:4836013-4837545question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000268231 ENST00000396693

Gene Information

This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis exocytosis embryonic development and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   SEPT12
Gene Name:   septin 12
Aliases:   FLJ25410
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000140623
Entrez:   124404
UniGene:   Hs.126780
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.390000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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