PrimePCR™ SYBR® Green Assay: GTPBP8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030606

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016461
Assay Design:   Intron-spanning
Chromosome Location:   3:112715739-112718366question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000383678 ENST00000383677 ENST00000467752 ENST00000473129 ENST00000305485

Gene Information

Description Not Available

Gene Symbol:   GTPBP8
Gene Name:   GTP-binding protein 8 (putative)
Aliases:   HSPC135
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000163607
Entrez:   29083
UniGene:   Hs.127496
Chromosome Mapping:   3q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.520000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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