This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030567
PrimePCR™ PreAmp for SYBR® Green Assay: MAP2K6, Human
PrimePCR™ Template for SYBR® Green Assay: MAP2K6, Human
This gene encodes a member of the dual specificity protein kinase family which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway this gene is involved in many cellular processes such as stress induced cell cycle arrest transcription activation and apoptosis. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.