PrimePCR™ SYBR® Green Assay: ARMCX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030493

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016284
Assay Design:   Intron-spanning
Chromosome Location:   X:100806571-100807922question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000372829 ENST00000538894

Gene Information

This gene encodes a member of the ALEX family of proteins and may play a role in tumor suppression. The encoded protein contains a potential N-terminal transmembrane domain and two Armadillo (arm) repeats. Other proteins containing the arm repeat are involved in development maintenance of tissue integrity and tumorigenesis. This gene is closely localized with other family members including ALEX2 and ALEX3 on the X chromosome. [provided by RefSeq Jul 2008]

Gene Symbol:   ARMCX1
Gene Name:   armadillo repeat containing, X-linked 1
Aliases:   ALEX1, DKFZp686P06199
RefSeq:   NC_000023.10 NG_015846.1 NT_011651.17
Ensembl:   ENSG00000126947
Entrez:   51309
UniGene:   Hs.9728
Chromosome Mapping:   Xq21.33-q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.460000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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