PrimePCR™ SYBR® Green Assay: MKRN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016233
Assay Design:   Intron-spanning
Chromosome Location:   3:12616372-12618162question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000170447 ENST00000411987 ENST00000448482

Gene Information

Members of the makorin family including MKRN2 have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al. 2001 [PubMed 11597136]).[supplied by OMIM Mar 2008]

Gene Symbol:   MKRN2
Gene Name:   makorin ring finger protein 2
Aliases:   RNF62
RefSeq:   NC_000003.11 NG_007467.1 NT_022517.18
Ensembl:   ENSG00000075975
Entrez:   23609
UniGene:   Hs.591666
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.570000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download