PrimePCR™ SYBR® Green Assay: TBX4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030453

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016230
Assay Design:   Intron-spanning
Chromosome Location:   17:59543269-59544952question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000393853 ENST00000240335

Gene Information

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4 which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene like TBX2 maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb but not in forelimb buds suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq Jul 2008]

Gene Symbol:   TBX4
Gene Name:   T-box 4
Aliases:   SPS
RefSeq:   NC_000017.10 NG_008080.1 NT_010783.15
Ensembl:   ENSG00000121075
Entrez:   9496
UniGene:   Hs.143907
Chromosome Mapping:   17q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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