This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030453
PrimePCR™ PreAmp for SYBR® Green Assay: TBX4, Human
PrimePCR™ Template for SYBR® Green Assay: TBX4, Human
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4 which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene like TBX2 maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb but not in forelimb buds suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.