PrimePCR™ SYBR® Green Assay: SEPT4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030422

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEPT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEPT4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016182
Assay Design:   Intron-spanning
Chromosome Location:   17:56599384-56602531question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000412945 ENST00000457347 ENST00000317256 ENST00000317268 ENST00000393086 ENST00000426861

Gene Information

This gene is a member of the septin family of nucleotide binding proteins originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast Drosophila and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria and has a role in apoptosis and cancer. [provided by RefSeq Nov 2010]

Gene Symbol:   SEPT4
Gene Name:   septin 4
Aliases:   ARTS, BRADEION, CE5B3, H5, MART, PNUTL2, SEP4, hCDCREL-2
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108387
Entrez:   5414
Chromosome Mapping:   17q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 34.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download