This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030151
PrimePCR™ PreAmp for SYBR® Green Assay: GDF6, Human
PrimePCR™ Template for SYBR® Green Assay: GDF6, Human
This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs skull and axial skeleton. Mutations in this gene result in colobomata which are congenital abnormalities in ocular development and in Klippel-Feil syndrome (KFS) which is a congenital disorder of spinal segmentation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.