PrimePCR™ SYBR® Green Assay: VPS4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030055

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VPS4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VPS4A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015627
Assay Design:   Intron-spanning
Chromosome Location:   16:69356564-69358228question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000254950

Gene Information

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities) and is the homolog of the yeast Vps4 protein. In humans two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments and are involved in intracellular protein trafficking similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS4A
Gene Name:   vacuolar protein sorting 4 homolog A (S. cerevisiae)
Aliases:   FLJ22197, SKD1, SKD1A, SKD2, VPS4, VPS4-1
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000132612
Entrez:   27183
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.960000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download