This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency
PrimePCR™ PreAmp for SYBR® Green Assay: CXCL13, Human
PrimePCR™ Template for SYBR® Green Assay: CXCL13, Human
B lymphocyte chemoattractant independently cloned and named Angie is a CXC chemokine strongly expressed in the follicles of the spleen lymph nodes and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages) apparently by stimulating calcium influx into and chemotaxis of cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.