PrimePCR™ SYBR® Green Assay: ME1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029802

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ME1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ME1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015244
Assay Design:   Intron-spanning
Chromosome Location:   6:83921710-83926282question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000369705 ENST00000543031 ENST00000541327 ENST00000540036

Gene Information

This gene encodes a cytosolic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme the reversible oxidative decarboxylation of malate links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq Jul 2008]

Gene Symbol:   ME1
Gene Name:   malic enzyme 1, NADP(+)-dependent, cytosolic
Aliases:   HUMNDME, MES
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000065833
Entrez:   4199
Chromosome Mapping:   6q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 33.800000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download